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Do you ever have so much to do that it seems overwhelming and you just don't want to do any of it? I'm having one of those days.
I read a story over on pointlesswasteoftime.com. It's good. It's part of John Dies At The End. I'll have to read the first part. Those guys could be professional writers. It's better than sex-typing 200 fish. Not by sequencing either, no, that would be too easy. Nor by using our awesome realtime PCR machine, because we'd have to buy an expensive-ass assay and it would be way too easy. No, I have to run every sample on a gel - it's like amelogenin, you get one band for females and two for males. Also, I have to get these three remaining human Y SNP primers to freaking work. Before I kill someone. I just tried it with different concentrations of template. No good. I'm thinking of trying it with a ton of template next, in case I just have crappy DNA for these individuals. I'd qualtify it using the RT machine - I can do that now - except for that to work, I need primers that'll amplify it (we're using SYBR Green, so it doesn't matter what primers I use but I need some that I know will work... hmm, maybe amelogenin. That's pretty reliable. Except I'm using up more and more template.) I should try the primers with known good DNA. Maybe I'll do that next.
Not to mention the miscelaneous fish samples I have four days left to get done and more keep coming in the mail. Two came in today. I hope those two aren't also due on Nov 19. That doesn't seem reasonable. I'd better take them in for sequencing tomorrow. And hope that the sequences come back good. Damnit, this always happens. I get stuck not being able to do things far enough in advance that I have time to send them for sequencing a second time in case they don't work the first time.
Is anyone still reading this? Sorry. I just have to complain about this stuff, you know?
I read a story over on pointlesswasteoftime.com. It's good. It's part of John Dies At The End. I'll have to read the first part. Those guys could be professional writers. It's better than sex-typing 200 fish. Not by sequencing either, no, that would be too easy. Nor by using our awesome realtime PCR machine, because we'd have to buy an expensive-ass assay and it would be way too easy. No, I have to run every sample on a gel - it's like amelogenin, you get one band for females and two for males. Also, I have to get these three remaining human Y SNP primers to freaking work. Before I kill someone. I just tried it with different concentrations of template. No good. I'm thinking of trying it with a ton of template next, in case I just have crappy DNA for these individuals. I'd qualtify it using the RT machine - I can do that now - except for that to work, I need primers that'll amplify it (we're using SYBR Green, so it doesn't matter what primers I use but I need some that I know will work... hmm, maybe amelogenin. That's pretty reliable. Except I'm using up more and more template.) I should try the primers with known good DNA. Maybe I'll do that next.
Not to mention the miscelaneous fish samples I have four days left to get done and more keep coming in the mail. Two came in today. I hope those two aren't also due on Nov 19. That doesn't seem reasonable. I'd better take them in for sequencing tomorrow. And hope that the sequences come back good. Damnit, this always happens. I get stuck not being able to do things far enough in advance that I have time to send them for sequencing a second time in case they don't work the first time.
Is anyone still reading this? Sorry. I just have to complain about this stuff, you know?