dur

[gmonkey42]

Aw, man. Yesterday, I tried to run my gel and nothing showed up. I assumed I'd just run it too far and the DNA went off the end. So I tried it again today. Still nothing. I came to J___ for help. He pointed out that the ethidium bromide hadn't moved either (it runs in the opposite direction to the DNA), which I hadn't noticed, though the power source definitely said there was current. He said to change the buffer and try it again to see if that would fix it. We put the gel into the tray with fresh buffer and went to plug it in... and the power source was plugged into a different tray from the one I'd been using. So there was current, but it was going through the other tray. So we had a good laugh. And I feel dumb. But that sort of thing happens to everyone sometimes so I don't feel too bad.